Mechanisms and new therapeutic strategies for post-infarction arrhythmias
1 Purpose
Our goal is to understand mechanisms that result in ventricular arrhythmias after myocardial infarction (MI), and to identify new targets for therapy. We want to study electrical remodeling and arrhythmias that occur up to 7 days after MI, and after repeat MI.
2 Distress
Some animals in the project will be subjected to procedures of moderate severity, i.e. three separate procedures that involve general anesthesia and percutaneous invasive procedures with some expected post-operative pain.
3 Expected benefit
This project will result in improved understanding of the mechanisms underlying arrhythmias that occur after MI. We will also test therapeutic strategies that might be brought into clinical testing.
4 Number of animals, and what kind
90 pigs
5 How to adhere to 3R
The project is based on previous publications from our institution, and is planned in parallel with another comprehensive project using rodents to establish key concepts. This will enable us to limit experiments with pigs to the minimum needed for validation of key results, where this cannot be gained from replacement by other models. All personnel are chosen for their procedural skills in order to ensure efficient experiments based on previous refinement of the procedures, and thereby maximum reduction in the number of animals needed.
Our goal is to understand mechanisms that result in ventricular arrhythmias after myocardial infarction (MI), and to identify new targets for therapy. We want to study electrical remodeling and arrhythmias that occur up to 7 days after MI, and after repeat MI.
2 Distress
Some animals in the project will be subjected to procedures of moderate severity, i.e. three separate procedures that involve general anesthesia and percutaneous invasive procedures with some expected post-operative pain.
3 Expected benefit
This project will result in improved understanding of the mechanisms underlying arrhythmias that occur after MI. We will also test therapeutic strategies that might be brought into clinical testing.
4 Number of animals, and what kind
90 pigs
5 How to adhere to 3R
The project is based on previous publications from our institution, and is planned in parallel with another comprehensive project using rodents to establish key concepts. This will enable us to limit experiments with pigs to the minimum needed for validation of key results, where this cannot be gained from replacement by other models. All personnel are chosen for their procedural skills in order to ensure efficient experiments based on previous refinement of the procedures, and thereby maximum reduction in the number of animals needed.